Thymosin beta-4 is a 43-residue peptide that binds monomeric G-actin and regulates the pool available for filament assembly. That role places it at the heart of in-vitro cytoskeleton and migration research.
The actin-binding motif
A central LKKTET sequence is key to actin interaction. Synthetic sequences such as TB-500 allow researchers to study this behaviour with a defined, high-purity reference material.
Common migration assays
- Scratch (wound-closure) assays imaged over time
- Transwell / Boyden chamber migration
- Live-cell imaging of lamellipodia formation
- Actin polymerisation assays with pyrene-labelled actin
Controls
Use mitomycin C or serum starvation to separate migration from proliferation, and include vehicle controls on each plate.
Related reagents: TB-500 10 mg. Each batch ships with documented HPLC purity and a Certificate of Analysis on request.
