Nicotinamide adenine dinucleotide exists in oxidised (NAD+) and reduced (NADH) forms and shuttles electrons in hundreds of enzymatic reactions. As a reagent it is essential to dehydrogenase, sirtuin and PARP assays.
Key research applications
- Dehydrogenase kinetics followed by NADH absorbance at 340 nm
- Sirtuin deacetylase assays, where NAD+ is a consumed co-substrate
- PARP activity assays measuring ADP-ribosylation
- Coupled enzyme systems in metabolic research
Stability and handling
NAD+ is relatively stable as a dry powder but degrades in alkaline solution. Prepare solutions fresh in a neutral to slightly acidic buffer, keep them on ice and freeze aliquots for later use.
Related reagents: NAD+ 500 mg laboratory reagent. Each batch ships with documented HPLC purity and a Certificate of Analysis on request.
