Repeated freezing and thawing promotes aggregation, ice-interface denaturation and concentration changes from evaporation. The simplest fix is to divide a reconstituted peptide into single-use portions.
A simple aliquoting workflow
- Equilibrate the lyophilised vial to room temperature.
- Reconstitute aseptically and swirl gently until dissolved.
- Dispense single-experiment volumes into low-binding tubes.
- Label with compound, concentration, lot and date.
- Freeze quickly and store at −20 °C or below.
Choosing tubes
Low-protein-binding polypropylene minimises losses for hydrophobic and lipidated peptides. Avoid glass for very dilute solutions unless silanised.
Related reagents: BAC water and peptide reagents. Each batch ships with documented HPLC purity and a Certificate of Analysis on request.
